Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4199301..4199877 Replicon chromosome
Accession NZ_CP121153
Organism Escherichia coli strain E57

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag P6H84_RS20430 Protein ID WP_001131965.1
Coordinates 4199590..4199877 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag P6H84_RS20425 Protein ID WP_000063158.1
Coordinates 4199301..4199603 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H84_RS20405 (4194409) 4194409..4195629 + 1221 WP_000873756.1 amino acid deaminase -
P6H84_RS20410 (4195897) 4195897..4198047 + 2151 WP_267657476.1 pyruvate/proton symporter BtsT -
P6H84_RS20415 (4198097) 4198097..4198300 + 204 WP_000467859.1 YbdD/YjiX family protein -
P6H84_RS20420 (4198311) 4198311..4199267 + 957 WP_001361361.1 GTPase -
P6H84_RS20425 (4199301) 4199301..4199603 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
P6H84_RS20430 (4199590) 4199590..4199877 - 288 WP_001131965.1 BrnT family toxin Toxin
P6H84_RS20435 (4200074) 4200074..4201240 + 1167 WP_000800823.1 restriction endonuclease -
P6H84_RS20440 (4201285) 4201285..4201458 + 174 Protein_4003 type I restriction-modification system subunit M N-terminal domain-containing protein -
P6H84_RS20445 (4201452) 4201452..4202120 + 669 Protein_4004 DUF3387 domain-containing protein -
P6H84_RS20450 (4202335) 4202335..4202673 + 339 WP_000132633.1 endoribonuclease SymE -
P6H84_RS20455 (4202761) 4202761..4202808 - 48 WP_229017048.1 hypothetical protein -
P6H84_RS20460 (4203056) 4203056..4204468 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T275941 WP_001131965.1 NZ_CP121153:c4199877-4199590 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT275941 WP_000063158.1 NZ_CP121153:c4199603-4199301 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References