Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 31539..32086 Replicon plasmid p12579_2
Accession NC_017657
Organism Escherichia coli O55:H7 str. RM12579

Toxin (Protein)


Gene name parE Uniprot ID A0A896ZGX1
Locus tag ECO55CA74_RS27170 Protein ID WP_000969997.1
Coordinates 31805..32086 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A7U8WKC6
Locus tag ECO55CA74_RS27165 Protein ID WP_000079946.1
Coordinates 31539..31808 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO55CA74_RS27130 27241..27756 + 516 WP_000998852.1 type II secretion system protein M -
ECO55CA74_RS27135 27808..28641 + 834 WP_000088803.1 A24 family peptidase -
ECO55CA74_RS27140 28735..29136 + 402 WP_001222321.1 GspS family T2SS pilot lipoprotein variant EptO -
ECO55CA74_RS30750 29211..29426 + 216 Protein_38 IS3 family transposase -
ECO55CA74_RS31930 29725..30018 + 294 Protein_39 hypothetical protein -
ECO55CA74_RS27155 30247..30495 + 249 WP_000083841.1 replication regulatory protein RepA -
ECO55CA74_RS32070 30518..30625 + 108 Protein_41 incFII family plasmid replication initiator RepA -
ECO55CA74_RS31935 31326..31484 + 159 WP_000829588.1 hypothetical protein -
ECO55CA74_RS27165 31539..31808 + 270 WP_000079946.1 hypothetical protein Antitoxin
ECO55CA74_RS27170 31805..32086 + 282 WP_000969997.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECO55CA74_RS27175 32282..32623 - 342 WP_000578011.1 hypothetical protein -
ECO55CA74_RS27180 32923..33510 + 588 WP_001418189.1 serine acetyltransferase -
ECO55CA74_RS27185 33792..34385 - 594 WP_000517696.1 hypothetical protein -
ECO55CA74_RS27190 34739..36085 + 1347 WP_001132032.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - stcE / exeE / exeG / nleA/espI 1..66078 66078


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10879.54 Da        Isoelectric Point: 8.4615

>T27594 WP_000969997.1 NC_017657:31805-32086 [Escherichia coli O55:H7 str. RM12579]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YVLRVLHTAQKWP

Download         Length: 282 bp

>T27594 NC_017657:31805-32086 [Escherichia coli O55:H7 str. RM12579]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTC
GTGTGGAAGGAACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATGTCCTGCGCGTGTTGCATACCGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10210.55 Da        Isoelectric Point: 8.8735

>AT27594 WP_000079946.1 NC_017657:31539-31808 [Escherichia coli O55:H7 str. RM12579]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSATAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT27594 NC_017657:31539-31808 [Escherichia coli O55:H7 str. RM12579]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGTGGTGATGTGCTTCCACATGACATGGTCGAATCCTCCGCGACTGCATGGCGGGAT
GAAATGAGCAGAAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A896ZGX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U8WKC6

References