Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4540215..4540791 Replicon chromosome
Accession NZ_CP121151
Organism Escherichia coli strain E47

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag P6H31_RS22480 Protein ID WP_001295743.1
Coordinates 4540504..4540791 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag P6H31_RS22475 Protein ID WP_000063148.1
Coordinates 4540215..4540517 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H31_RS22460 (4536811) 4536811..4538961 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
P6H31_RS22465 (4539011) 4539011..4539214 + 204 WP_000467859.1 YbdD/YjiX family protein -
P6H31_RS22470 (4539225) 4539225..4540181 + 957 WP_001295745.1 GTPase -
P6H31_RS22475 (4540215) 4540215..4540517 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
P6H31_RS22480 (4540504) 4540504..4540791 - 288 WP_001295743.1 BrnT family toxin Toxin
P6H31_RS22485 (4540988) 4540988..4542154 + 1167 WP_000800831.1 restriction endonuclease -
P6H31_RS22490 (4542218) 4542218..4543837 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
P6H31_RS22495 (4543827) 4543827..4545131 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T275916 WP_001295743.1 NZ_CP121151:c4540791-4540504 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT275916 WP_000063148.1 NZ_CP121151:c4540517-4540215 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References