Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4015841..4016678 Replicon chromosome
Accession NZ_CP121151
Organism Escherichia coli strain E47

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag P6H31_RS20065 Protein ID WP_000227784.1
Coordinates 4016136..4016678 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag P6H31_RS20060 Protein ID WP_001297137.1
Coordinates 4015841..4016152 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H31_RS20035 (4010861) 4010861..4011808 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
P6H31_RS20040 (4011830) 4011830..4013821 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
P6H31_RS20045 (4013811) 4013811..4014425 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
P6H31_RS20050 (4014425) 4014425..4014754 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
P6H31_RS20055 (4014766) 4014766..4015656 + 891 WP_000971336.1 heme o synthase -
P6H31_RS20060 (4015841) 4015841..4016152 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
P6H31_RS20065 (4016136) 4016136..4016678 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
P6H31_RS20070 (4016734) 4016734..4017669 - 936 WP_001297127.1 tetratricopeptide repeat protein -
P6H31_RS20075 (4018077) 4018077..4019441 + 1365 WP_001000978.1 MFS transporter -
P6H31_RS20080 (4019569) 4019569..4020060 - 492 WP_001138904.1 nucleotide binding protein YajQ -
P6H31_RS20085 (4020228) 4020228..4021139 + 912 WP_000705847.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T275914 WP_000227784.1 NZ_CP121151:4016136-4016678 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT275914 WP_001297137.1 NZ_CP121151:4015841-4016152 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References