Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4156623..4157254 Replicon chromosome
Accession NC_017656
Organism Escherichia coli O55:H7 str. RM12579

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag ECO55CA74_RS21020 Protein ID WP_001259385.1
Coordinates 4156979..4157254 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ECO55CA74_RS21015 Protein ID WP_000593555.1
Coordinates 4156623..4156982 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO55CA74_RS20985 4151735..4152208 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
ECO55CA74_RS20990 4152205..4152486 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
ECO55CA74_RS20995 4152563..4153921 + 1359 WP_001419219.1 PTS galactitol transporter subunit IIC -
ECO55CA74_RS21000 4153914..4155422 + 1509 WP_001301773.1 carbohydrate kinase -
ECO55CA74_RS21005 4155412..4155681 + 270 WP_000933060.1 HPr family phosphocarrier protein -
ECO55CA74_RS21010 4155713..4156573 + 861 WP_001131788.1 class II aldolase -
ECO55CA74_RS21015 4156623..4156982 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ECO55CA74_RS21020 4156979..4157254 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
ECO55CA74_RS21025 4157327..4158451 - 1125 WP_001301659.1 ABC transporter permease -
ECO55CA74_RS21030 4158451..4161186 - 2736 Protein_3975 ribosome-associated ATPase/putative transporter RbbA -
ECO55CA74_RS21035 4161183..4162250 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T27591 WP_001259385.1 NC_017656:c4157254-4156979 [Escherichia coli O55:H7 str. RM12579]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T27591 NC_017656:c4157254-4156979 [Escherichia coli O55:H7 str. RM12579]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT27591 WP_000593555.1 NC_017656:c4156982-4156623 [Escherichia coli O55:H7 str. RM12579]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT27591 NC_017656:c4156982-4156623 [Escherichia coli O55:H7 str. RM12579]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References