Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 77543..79166 Replicon plasmid pE14-1
Accession NZ_CP121150
Organism Escherichia coli strain E14

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag P1I99_RS24600 Protein ID WP_000088046.1
Coordinates 77543..78868 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag P1I99_RS24605 Protein ID WP_024136327.1
Coordinates 78873..79166 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1I99_RS24555 (P1I99_24555) 73199..73312 + 114 Protein_74 hypothetical protein -
P1I99_RS24560 (P1I99_24560) 73398..73628 - 231 Protein_75 aminoglycoside phosphotransferase family protein -
P1I99_RS24565 (P1I99_24565) 73680..74384 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
P1I99_RS24570 (P1I99_24570) 74651..75001 + 351 WP_000454193.1 DUF3330 domain-containing protein -
P1I99_RS24575 (P1I99_24575) 75127..75702 + 576 WP_072740834.1 recombinase family protein -
P1I99_RS24580 (P1I99_24580) 75749..76453 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
P1I99_RS24585 (P1I99_24585) 76506..76751 - 246 Protein_80 HNH endonuclease -
P1I99_RS24590 (P1I99_24590) 76764..77174 - 411 WP_000985911.1 hypothetical protein -
P1I99_RS24595 (P1I99_24595) 77276..77482 - 207 WP_000134171.1 hypothetical protein -
P1I99_RS24600 (P1I99_24600) 77543..78868 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
P1I99_RS24605 (P1I99_24605) 78873..79166 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
P1I99_RS24610 (P1I99_24610) 79770..80162 - 393 WP_000341066.1 hypothetical protein -
P1I99_RS24615 (P1I99_24615) 80535..80876 + 342 WP_001151575.1 hypothetical protein -
P1I99_RS24620 (P1I99_24620) 80968..81159 + 192 WP_000398480.1 hypothetical protein -
P1I99_RS24625 (P1I99_24625) 81209..81490 + 282 WP_000077926.1 hypothetical protein -
P1I99_RS24630 (P1I99_24630) 81831..81962 - 132 WP_000374059.1 hypothetical protein -
P1I99_RS24635 (P1I99_24635) 82053..83294 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / qnrS1 / tet(A) / blaTEM-1B / aadA2 / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / mcr-1.1 - 1..226260 226260


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T275896 WP_000088046.1 NZ_CP121150:c78868-77543 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT275896 WP_024136327.1 NZ_CP121150:c79166-78873 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References