Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4508825..4509322 Replicon chromosome
Accession NZ_CP121149
Organism Escherichia coli strain E14

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag P1I99_RS21960 Protein ID WP_001173343.1
Coordinates 4508825..4508998 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag P1I99_RS21965 Protein ID WP_000398619.1
Coordinates 4509026..4509322 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1I99_RS21950 (4505495) 4505495..4506952 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
P1I99_RS21955 (4507189) 4507189..4508706 + 1518 WP_001295074.1 lysine--tRNA ligase -
P1I99_RS21960 (4508825) 4508825..4508998 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
P1I99_RS21965 (4509026) 4509026..4509322 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
P1I99_RS21970 (4509549) 4509549..4509821 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
P1I99_RS21975 (4509833) 4509833..4510063 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
P1I99_RS21980 (4510244) 4510244..4511875 + 1632 WP_001216487.1 sensor histidine kinase -
P1I99_RS21985 (4511872) 4511872..4512591 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-55 papB / papI 4432047..4509322 77275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T275894 WP_001173343.1 NZ_CP121149:c4508998-4508825 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT275894 WP_000398619.1 NZ_CP121149:c4509322-4509026 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References