Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 925995..926649 Replicon chromosome
Accession NZ_CP121149
Organism Escherichia coli strain E14

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag P1I99_RS04525 Protein ID WP_000244772.1
Coordinates 926242..926649 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag P1I99_RS04520 Protein ID WP_000354046.1
Coordinates 925995..926261 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1I99_RS04495 (921283) 921283..922026 + 744 WP_000951964.1 SDR family oxidoreductase -
P1I99_RS04500 (922083) 922083..923516 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
P1I99_RS04505 (923561) 923561..923872 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
P1I99_RS04510 (924036) 924036..924695 + 660 WP_000250274.1 hemolysin III family protein -
P1I99_RS04515 (924772) 924772..925752 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
P1I99_RS04520 (925995) 925995..926261 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
P1I99_RS04525 (926242) 926242..926649 + 408 WP_000244772.1 protein YgfX Toxin
P1I99_RS04530 (926689) 926689..927210 - 522 WP_001055874.1 flavodoxin FldB -
P1I99_RS04535 (927322) 927322..928218 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
P1I99_RS04540 (928243) 928243..928953 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
P1I99_RS04545 (928959) 928959..930692 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T275880 WP_000244772.1 NZ_CP121149:926242-926649 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T275880 NZ_CP121149:926242-926649 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT275880 WP_000354046.1 NZ_CP121149:925995-926261 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT275880 NZ_CP121149:925995-926261 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References