Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 288572..289203 Replicon chromosome
Accession NZ_CP121149
Organism Escherichia coli strain E14

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag P1I99_RS01285 Protein ID WP_001259388.1
Coordinates 288572..288847 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag P1I99_RS01290 Protein ID WP_000593555.1
Coordinates 288844..289203 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1I99_RS01270 (283576) 283576..284643 + 1068 WP_000361485.1 HlyD family secretion protein -
P1I99_RS01275 (284640) 284640..287375 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
P1I99_RS01280 (287375) 287375..288499 + 1125 WP_001314210.1 ABC transporter permease -
P1I99_RS01285 (288572) 288572..288847 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
P1I99_RS01290 (288844) 288844..289203 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
P1I99_RS01295 (289323) 289323..289724 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
P1I99_RS01300 (289730) 289730..290536 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
P1I99_RS01305 (290533) 290533..291297 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
P1I99_RS01310 (291297) 291297..292130 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
P1I99_RS01315 (292127) 292127..293071 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T275877 WP_001259388.1 NZ_CP121149:288572-288847 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT275877 WP_000593555.1 NZ_CP121149:288844-289203 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References