Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 69017..69660 Replicon plasmid pE20-1
Accession NZ_CP121148
Organism Escherichia coli strain E20

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag P6H07_RS24175 Protein ID WP_001044768.1
Coordinates 69244..69660 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag P6H07_RS24170 Protein ID WP_001261287.1
Coordinates 69017..69247 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H07_RS24150 (64177) 64177..65265 + 1089 WP_000952217.1 transcriptional repressor PifC -
P6H07_RS24155 (65267) 65267..67492 + 2226 WP_000698737.1 P-loop NTPase fold protein -
P6H07_RS24160 (67542) 67542..68441 - 900 WP_000963206.1 nucleotide-binding protein -
P6H07_RS24165 (68431) 68431..68721 - 291 WP_000111771.1 hypothetical protein -
P6H07_RS24170 (69017) 69017..69247 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
P6H07_RS24175 (69244) 69244..69660 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
P6H07_RS24180 (69822) 69822..71960 - 2139 WP_000350638.1 AAA family ATPase -
P6H07_RS24185 (72314) 72314..72571 + 258 WP_000343085.1 hypothetical protein -
P6H07_RS24190 (72571) 72571..73161 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / tet(A) / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / blaCTX-M-27 / dfrA14 - 1..124410 124410


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T275876 WP_001044768.1 NZ_CP121148:69244-69660 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT275876 WP_001261287.1 NZ_CP121148:69017-69247 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References