Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4705639..4706255 Replicon chromosome
Accession NZ_CP121147
Organism Escherichia coli strain E20

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag P6H07_RS22695 Protein ID WP_001129490.1
Coordinates 4705639..4706013 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag P6H07_RS22700 Protein ID WP_001275523.1
Coordinates 4706013..4706255 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H07_RS22680 (4703142) 4703142..4704044 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
P6H07_RS22685 (4704041) 4704041..4704676 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
P6H07_RS22690 (4704673) 4704673..4705602 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
P6H07_RS22695 (4705639) 4705639..4706013 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
P6H07_RS22700 (4706013) 4706013..4706255 - 243 WP_001275523.1 CopG family transcriptional regulator Antitoxin
P6H07_RS22705 (4706475) 4706475..4706693 - 219 WP_001251290.1 CopG family transcriptional regulator -
P6H07_RS22710 (4707367) 4707367..4708281 - 915 WP_072649161.1 transposase -
P6H07_RS22715 (4708294) 4708294..4709181 - 888 Protein_4442 hypothetical protein -
P6H07_RS22720 (4709597) 4709597..4710538 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
P6H07_RS22725 (4710583) 4710583..4711020 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T275872 WP_001129490.1 NZ_CP121147:c4706013-4705639 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT275872 WP_001275523.1 NZ_CP121147:c4706255-4706013 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References