Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4179782..4180358 Replicon chromosome
Accession NZ_CP121147
Organism Escherichia coli strain E20

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag P6H07_RS20255 Protein ID WP_001131965.1
Coordinates 4180071..4180358 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag P6H07_RS20250 Protein ID WP_000063158.1
Coordinates 4179782..4180084 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H07_RS20230 (4174890) 4174890..4176110 + 1221 WP_000873756.1 amino acid deaminase -
P6H07_RS20235 (4176378) 4176378..4178528 + 2151 WP_267657476.1 pyruvate/proton symporter BtsT -
P6H07_RS20240 (4178578) 4178578..4178781 + 204 WP_000467859.1 YbdD/YjiX family protein -
P6H07_RS20245 (4178792) 4178792..4179748 + 957 WP_001361361.1 GTPase -
P6H07_RS20250 (4179782) 4179782..4180084 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
P6H07_RS20255 (4180071) 4180071..4180358 - 288 WP_001131965.1 BrnT family toxin Toxin
P6H07_RS20260 (4180555) 4180555..4181721 + 1167 WP_000800823.1 restriction endonuclease -
P6H07_RS20265 (4181766) 4181766..4181939 + 174 Protein_3969 type I restriction-modification system subunit M N-terminal domain-containing protein -
P6H07_RS20270 (4181933) 4181933..4182601 + 669 Protein_3970 DUF3387 domain-containing protein -
P6H07_RS20275 (4182816) 4182816..4183154 + 339 WP_000132633.1 endoribonuclease SymE -
P6H07_RS20280 (4183242) 4183242..4183289 - 48 WP_229017048.1 hypothetical protein -
P6H07_RS20285 (4183537) 4183537..4184949 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T275868 WP_001131965.1 NZ_CP121147:c4180358-4180071 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT275868 WP_000063158.1 NZ_CP121147:c4180084-4179782 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References