27586

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3516632..3517286 Replicon chromosome
Accession NC_017656
Organism Escherichia coli O55:H7 str. RM12579

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag ECO55CA74_RS17675 Protein ID WP_000244781.1
Coordinates 3516632..3517039 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECO55CA74_RS17680 Protein ID WP_000354046.1
Coordinates 3517020..3517286 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO55CA74_RS17655 3512589..3514322 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
ECO55CA74_RS17660 3514328..3515038 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECO55CA74_RS17665 3515063..3515959 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECO55CA74_RS17670 3516071..3516592 + 522 WP_001055874.1 flavodoxin FldB -
ECO55CA74_RS17675 3516632..3517039 - 408 WP_000244781.1 protein YgfX Toxin
ECO55CA74_RS17680 3517020..3517286 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECO55CA74_RS17685 3517529..3518509 + 981 WP_000886055.1 tRNA-modifying protein YgfZ -
ECO55CA74_RS17690 3518586..3519245 - 660 WP_000250274.1 hemolysin III family protein -
ECO55CA74_RS17695 3519409..3519720 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ECO55CA74_RS17700 3519765..3521198 + 1434 WP_001419180.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T27586 WP_000244781.1 NC_017656:c3517039-3516632 [Escherichia coli O55:H7 str. RM12579]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T27586 NC_017656:c3517039-3516632 [Escherichia coli O55:H7 str. RM12579]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT27586 WP_000354046.1 NC_017656:c3517286-3517020 [Escherichia coli O55:H7 str. RM12579]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT27586 NC_017656:c3517286-3517020 [Escherichia coli O55:H7 str. RM12579]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QD57

References