Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3385294..3385877 Replicon chromosome
Accession NC_017656
Organism Escherichia coli O55:H7 str. RM12579

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag ECO55CA74_RS17100 Protein ID WP_000254738.1
Coordinates 3385294..3385629 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag ECO55CA74_RS17105 Protein ID WP_000581937.1
Coordinates 3385629..3385877 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO55CA74_RS17085 3381181..3382479 - 1299 WP_000036723.1 phosphopyruvate hydratase -
ECO55CA74_RS17090 3382567..3384204 - 1638 WP_000210874.1 CTP synthase (glutamine hydrolyzing) -
ECO55CA74_RS17095 3384432..3385223 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
ECO55CA74_RS17100 3385294..3385629 - 336 WP_000254738.1 endoribonuclease MazF Toxin
ECO55CA74_RS17105 3385629..3385877 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
ECO55CA74_RS17110 3385955..3388189 - 2235 WP_000226815.1 GTP pyrophosphokinase -
ECO55CA74_RS17115 3388237..3389538 - 1302 WP_000046824.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T27585 WP_000254738.1 NC_017656:c3385629-3385294 [Escherichia coli O55:H7 str. RM12579]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T27585 NC_017656:c3385629-3385294 [Escherichia coli O55:H7 str. RM12579]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT27585 WP_000581937.1 NC_017656:c3385877-3385629 [Escherichia coli O55:H7 str. RM12579]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT27585 NC_017656:c3385877-3385629 [Escherichia coli O55:H7 str. RM12579]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References