Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4025035..4025660 Replicon chromosome
Accession NZ_CP121143
Organism Klebsiella pneumoniae strain UHD-57_PH

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag P8T33_RS19395 Protein ID WP_019705794.1
Coordinates 4025035..4025418 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag P8T33_RS19400 Protein ID WP_004150355.1
Coordinates 4025418..4025660 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P8T33_RS19380 (P8T33_19375) 4022401..4023303 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
P8T33_RS19385 (P8T33_19380) 4023300..4023935 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
P8T33_RS19390 (P8T33_19385) 4023932..4024861 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
P8T33_RS19395 (P8T33_19390) 4025035..4025418 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
P8T33_RS19400 (P8T33_19395) 4025418..4025660 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
P8T33_RS19405 (P8T33_19400) 4025865..4026782 + 918 WP_012737090.1 alpha/beta hydrolase -
P8T33_RS19410 (P8T33_19405) 4026796..4027737 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
P8T33_RS19415 (P8T33_19410) 4027782..4028219 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
P8T33_RS19420 (P8T33_19415) 4028216..4029076 - 861 WP_004146232.1 virulence factor BrkB family protein -
P8T33_RS19425 (P8T33_19420) 4029070..4029669 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T275831 WP_019705794.1 NZ_CP121143:c4025418-4025035 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT275831 WP_004150355.1 NZ_CP121143:c4025660-4025418 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References