Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3428579..3429389 Replicon chromosome
Accession NZ_CP121143
Organism Klebsiella pneumoniae strain UHD-57_PH

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag P8T33_RS16605 Protein ID WP_004178461.1
Coordinates 3428579..3429112 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag P8T33_RS16610 Protein ID WP_002887278.1
Coordinates 3429123..3429389 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P8T33_RS16600 (P8T33_16595) 3427410..3428531 + 1122 WP_012737335.1 cupin domain-containing protein -
P8T33_RS16605 (P8T33_16600) 3428579..3429112 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
P8T33_RS16610 (P8T33_16605) 3429123..3429389 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
P8T33_RS16615 (P8T33_16610) 3429492..3430925 - 1434 WP_032415907.1 Cu(+)/Ag(+) sensor histidine kinase -
P8T33_RS16620 (P8T33_16615) 3430915..3431598 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
P8T33_RS16625 (P8T33_16620) 3431771..3433156 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
P8T33_RS16630 (P8T33_16625) 3433174..3433518 + 345 WP_116288284.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T275828 WP_004178461.1 NZ_CP121143:c3429112-3428579 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT275828 WP_002887278.1 NZ_CP121143:c3429389-3429123 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References