Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1296305..1296930 Replicon chromosome
Accession NZ_CP121141
Organism Klebsiella pneumoniae strain UHD-58_PH

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag P8T47_RS06405 Protein ID WP_019705794.1
Coordinates 1296547..1296930 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag P8T47_RS06400 Protein ID WP_004150355.1
Coordinates 1296305..1296547 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P8T47_RS06375 (P8T47_06375) 1292296..1292895 + 600 WP_004151865.1 glucose-1-phosphatase -
P8T47_RS06380 (P8T47_06380) 1292889..1293749 + 861 WP_004146232.1 virulence factor BrkB family protein -
P8T47_RS06385 (P8T47_06385) 1293746..1294183 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
P8T47_RS06390 (P8T47_06390) 1294228..1295169 + 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
P8T47_RS06395 (P8T47_06395) 1295183..1296100 - 918 WP_012737090.1 alpha/beta hydrolase -
P8T47_RS06400 (P8T47_06400) 1296305..1296547 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
P8T47_RS06405 (P8T47_06405) 1296547..1296930 + 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
P8T47_RS06410 (P8T47_06410) 1297104..1298033 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
P8T47_RS06415 (P8T47_06415) 1298030..1298665 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
P8T47_RS06420 (P8T47_06420) 1298662..1299564 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T275811 WP_019705794.1 NZ_CP121141:1296547-1296930 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT275811 WP_004150355.1 NZ_CP121141:1296305-1296547 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References