Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5102888..5103513 Replicon chromosome
Accession NZ_CP121139
Organism Klebsiella pneumoniae strain UHD-4_PH

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag P8T35_RS24740 Protein ID WP_019705794.1
Coordinates 5103130..5103513 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag P8T35_RS24735 Protein ID WP_004150355.1
Coordinates 5102888..5103130 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P8T35_RS24710 (P8T35_24710) 5098879..5099478 + 600 WP_004151865.1 glucose-1-phosphatase -
P8T35_RS24715 (P8T35_24715) 5099472..5100332 + 861 WP_004146232.1 virulence factor BrkB family protein -
P8T35_RS24720 (P8T35_24720) 5100329..5100766 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
P8T35_RS24725 (P8T35_24725) 5100811..5101752 + 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
P8T35_RS24730 (P8T35_24730) 5101766..5102683 - 918 WP_012737090.1 alpha/beta hydrolase -
P8T35_RS24735 (P8T35_24735) 5102888..5103130 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
P8T35_RS24740 (P8T35_24740) 5103130..5103513 + 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
P8T35_RS24745 (P8T35_24745) 5103687..5104616 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
P8T35_RS24750 (P8T35_24750) 5104613..5105248 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
P8T35_RS24755 (P8T35_24755) 5105245..5106147 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T275805 WP_019705794.1 NZ_CP121139:5103130-5103513 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT275805 WP_004150355.1 NZ_CP121139:5102888-5103130 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References