Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3466184..3466809 Replicon chromosome
Accession NZ_CP121131
Organism Klebsiella pneumoniae strain UHD-50_PH

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag P8T45_RS16795 Protein ID WP_019705794.1
Coordinates 3466184..3466567 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag P8T45_RS16800 Protein ID WP_004150355.1
Coordinates 3466567..3466809 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P8T45_RS16780 (P8T45_16785) 3463550..3464452 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
P8T45_RS16785 (P8T45_16790) 3464449..3465084 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
P8T45_RS16790 (P8T45_16795) 3465081..3466010 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
P8T45_RS16795 (P8T45_16800) 3466184..3466567 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
P8T45_RS16800 (P8T45_16805) 3466567..3466809 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
P8T45_RS16805 (P8T45_16810) 3467014..3467931 + 918 WP_012737090.1 alpha/beta hydrolase -
P8T45_RS16810 (P8T45_16815) 3467945..3468886 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
P8T45_RS16815 (P8T45_16820) 3468931..3469368 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
P8T45_RS16820 (P8T45_16825) 3469365..3470225 - 861 WP_004146232.1 virulence factor BrkB family protein -
P8T45_RS16825 (P8T45_16830) 3470219..3470818 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T275741 WP_019705794.1 NZ_CP121131:c3466567-3466184 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT275741 WP_004150355.1 NZ_CP121131:c3466809-3466567 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References