Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1824055..1824693 Replicon chromosome
Accession NC_017656
Organism Escherichia coli O55:H7 str. RM12579

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ECO55CA74_RS09185 Protein ID WP_000813794.1
Coordinates 1824055..1824231 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ECO55CA74_RS09190 Protein ID WP_001270286.1
Coordinates 1824277..1824693 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO55CA74_RS09165 1819677..1820888 - 1212 WP_071525382.1 BenE family transporter YdcO -
ECO55CA74_RS09170 1820941..1821477 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
ECO55CA74_RS09175 1821550..1823511 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ECO55CA74_RS09180 1823603..1823833 - 231 WP_000494244.1 YncJ family protein -
ECO55CA74_RS09185 1824055..1824231 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ECO55CA74_RS09190 1824277..1824693 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ECO55CA74_RS09195 1824772..1826178 + 1407 WP_000760653.1 PLP-dependent aminotransferase family protein -
ECO55CA74_RS09200 1826423..1827568 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
ECO55CA74_RS09205 1827586..1828599 + 1014 WP_000220401.1 ABC transporter ATP-binding protein -
ECO55CA74_RS09210 1828600..1829541 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T27574 WP_000813794.1 NC_017656:1824055-1824231 [Escherichia coli O55:H7 str. RM12579]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T27574 NC_017656:1824055-1824231 [Escherichia coli O55:H7 str. RM12579]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT27574 WP_001270286.1 NC_017656:1824277-1824693 [Escherichia coli O55:H7 str. RM12579]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT27574 NC_017656:1824277-1824693 [Escherichia coli O55:H7 str. RM12579]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References