Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2869729..2870539 Replicon chromosome
Accession NZ_CP121131
Organism Klebsiella pneumoniae strain UHD-50_PH

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag P8T45_RS14005 Protein ID WP_004178461.1
Coordinates 2869729..2870262 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag P8T45_RS14010 Protein ID WP_002887278.1
Coordinates 2870273..2870539 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P8T45_RS14000 (P8T45_14005) 2868560..2869681 + 1122 WP_012737335.1 cupin domain-containing protein -
P8T45_RS14005 (P8T45_14010) 2869729..2870262 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
P8T45_RS14010 (P8T45_14015) 2870273..2870539 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
P8T45_RS14015 (P8T45_14020) 2870642..2872075 - 1434 WP_032415907.1 Cu(+)/Ag(+) sensor histidine kinase -
P8T45_RS14020 (P8T45_14025) 2872065..2872748 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
P8T45_RS14025 (P8T45_14030) 2872921..2874306 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
P8T45_RS14030 (P8T45_14035) 2874324..2874668 + 345 WP_116288284.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T275738 WP_004178461.1 NZ_CP121131:c2870262-2869729 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT275738 WP_002887278.1 NZ_CP121131:c2870539-2870273 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References