275686

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 145386..146029 Replicon plasmid p167kb
Accession NZ_CP121088
Organism Escherichia coli strain ST3

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag P5670_RS24770 Protein ID WP_001034044.1
Coordinates 145613..146029 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag P5670_RS24765 Protein ID WP_001261286.1
Coordinates 145386..145616 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5670_RS24750 (140523) 140523..140753 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
P5670_RS24755 (140750) 140750..141166 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
P5670_RS24760 (141211) 141211..145005 - 3795 WP_001144731.1 hypothetical protein -
P5670_RS24765 (145386) 145386..145616 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
P5670_RS24770 (145613) 145613..146029 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
P5670_RS24775 (146104) 146104..147669 + 1566 WP_001128474.1 AAA family ATPase -
P5670_RS24780 (147654) 147654..148676 + 1023 WP_000361402.1 helicase UvrD -
P5670_RS24790 (149983) 149983..150897 + 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / qnrS1 / blaTEM-1B / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iucA / iucB / iucC / iucD / iutA 1..167740 167740
- flank IS/Tn sitABCD - 148930..153432 4502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T275686 WP_001034044.1 NZ_CP121088:145613-146029 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT275686 WP_001261286.1 NZ_CP121088:145386-145616 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References