Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4095461..4096115 Replicon chromosome
Accession NZ_CP121087
Organism Escherichia coli strain ST3

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag P5670_RS20015 Protein ID WP_000244781.1
Coordinates 4095708..4096115 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag P5670_RS20010 Protein ID WP_000354046.1
Coordinates 4095461..4095727 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5670_RS19985 (4090621) 4090621..4091364 + 744 WP_000951966.1 SDR family oxidoreductase -
P5670_RS19990 (4091421) 4091421..4092854 - 1434 WP_001605840.1 6-phospho-beta-glucosidase BglA -
P5670_RS19995 (4092899) 4092899..4093210 + 312 WP_001182943.1 N(4)-acetylcytidine aminohydrolase -
P5670_RS20000 (4093374) 4093374..4094033 + 660 WP_000250283.1 hemolysin III family protein -
P5670_RS20005 (4094229) 4094229..4095209 - 981 WP_000886102.1 tRNA-modifying protein YgfZ -
P5670_RS20010 (4095461) 4095461..4095727 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
P5670_RS20015 (4095708) 4095708..4096115 + 408 WP_000244781.1 protein YgfX Toxin
P5670_RS20020 (4096155) 4096155..4096676 - 522 WP_001055874.1 flavodoxin FldB -
P5670_RS20025 (4096788) 4096788..4097684 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
P5670_RS20030 (4097709) 4097709..4098419 + 711 WP_000715223.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
P5670_RS20035 (4098425) 4098425..4100158 + 1734 WP_000813232.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T275678 WP_000244781.1 NZ_CP121087:4095708-4096115 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT275678 WP_000354046.1 NZ_CP121087:4095461-4095727 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References