27565

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 54779..55329 Replicon chromosome
Accession NC_017656
Organism Escherichia coli O55:H7 str. RM12579

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag ECO55CA74_RS00270 Protein ID WP_001160975.1
Coordinates 55015..55329 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag ECO55CA74_RS00265 Protein ID WP_000125566.1
Coordinates 54779..55012 (+) Length 78 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO55CA74_RS00240 49853..50140 + 288 WP_000203741.1 ferredoxin-like protein FixX -
ECO55CA74_RS00245 50198..51529 + 1332 WP_001183177.1 MFS transporter -
ECO55CA74_RS00250 51637..52167 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
ECO55CA74_RS00255 52160..54022 + 1863 WP_000377186.1 glutathione-regulated potassium-efflux system protein KefC -
ECO55CA74_RS00260 54214..54693 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
ECO55CA74_RS00265 54779..55012 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
ECO55CA74_RS00270 55015..55329 + 315 WP_001160975.1 CcdB family protein Toxin
ECO55CA74_RS00275 55326..56174 - 849 WP_000257187.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
ECO55CA74_RS00280 56181..56558 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
ECO55CA74_RS00285 56561..57382 - 822 WP_001065380.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
ECO55CA74_RS00290 57379..58368 - 990 WP_000241259.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
ECO55CA74_RS00295 58368..59654 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-103)

Antitoxin

(8-77)


Sequences


Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T27565 WP_001160975.1 NC_017656:55015-55329 [Escherichia coli O55:H7 str. RM12579]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T27565 NC_017656:55015-55329 [Escherichia coli O55:H7 str. RM12579]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT27565 WP_000125566.1 NC_017656:54779-55012 [Escherichia coli O55:H7 str. RM12579]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT27565 NC_017656:54779-55012 [Escherichia coli O55:H7 str. RM12579]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References