Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 87260..87861 Replicon plasmid p12579_1
Accession NC_017653
Organism Escherichia coli O55:H7 str. RM12579

Toxin (Protein)


Gene name doc Uniprot ID U9Z1Q0
Locus tag ECO55CA74_RS26890 Protein ID WP_001216047.1
Coordinates 87481..87861 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag ECO55CA74_RS26885 Protein ID WP_001190712.1
Coordinates 87260..87481 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO55CA74_RS26840 82632..82922 + 291 WP_001018057.1 DUF4752 family protein -
ECO55CA74_RS26845 82919..83632 + 714 WP_000672529.1 hypothetical protein -
ECO55CA74_RS26850 83629..83928 + 300 WP_000215167.1 hypothetical protein -
ECO55CA74_RS26855 83930..84616 + 687 WP_000161573.1 ead/Ea22-like family protein -
ECO55CA74_RS32060 84618..84767 + 150 WP_001151811.1 hypothetical protein -
ECO55CA74_RS26860 84771..85787 + 1017 WP_000208040.1 hypothetical protein -
ECO55CA74_RS26865 85771..86055 + 285 WP_001142394.1 hypothetical protein -
ECO55CA74_RS29790 86040..86249 - 210 WP_001344848.1 hypothetical protein -
ECO55CA74_RS26875 86423..86674 + 252 WP_001283837.1 DNA polymerase III subunit theta -
ECO55CA74_RS26880 86798..87187 + 390 WP_000506726.1 DNA repair protein -
ECO55CA74_RS26885 87260..87481 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ECO55CA74_RS26890 87481..87861 + 381 WP_001216047.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ECO55CA74_RS26895 87866..88045 + 180 WP_000113019.1 hypothetical protein -
ECO55CA74_RS26900 88073..89116 + 1044 WP_000644102.1 DUF968 domain-containing protein -
ECO55CA74_RS26905 89205..89657 + 453 WP_001312282.1 late promoter-activating protein (Gp10) -
ECO55CA74_RS26910 89744..90937 + 1194 WP_000219608.1 hypothetical protein -
ECO55CA74_RS26915 90937..92421 + 1485 WP_000124150.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..94015 94015


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13574.27 Da        Isoelectric Point: 5.1514

>T27564 WP_001216047.1 NC_017653:87481-87861 [Escherichia coli O55:H7 str. RM12579]
MRHISPEELVALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T27564 NC_017653:87481-87861 [Escherichia coli O55:H7 str. RM12579]
ATGAGACATATATCACCGGAAGAACTTGTTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT27564 WP_001190712.1 NC_017653:87260-87481 [Escherichia coli O55:H7 str. RM12579]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT27564 NC_017653:87260-87481 [Escherichia coli O55:H7 str. RM12579]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Z1Q0

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References