Detailed information of TA system TA027554

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3364273..3365107
• Replicon: chromosome
• Accession: NC_017652
• Organism: Escherichia coli str. clone D i14

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: I14_RS16595
• Protein ID: WP_000854690.1
• Coordinates: 3364730..3365107 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: I14_RS16590
• Protein ID: WP_001305076.1
• Coordinates: 3364273..3364641 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I14_RS16550	3359355..3360482	+	1128	Protein_3126	hypothetical protein	-
I14_RS16555	3360558..3361013	+	456	WP_000581502.1	hypothetical protein	-
I14_RS16560	3361092..3361325	+	234	WP_000902034.1	DUF905 family protein	-
I14_RS16565	3361426..3362244	+	819	WP_001234620.1	DUF945 domain-containing protein	-
I14_RS16570	3362299..3362784	+	486	WP_000849565.1	antirestriction protein	-
I14_RS16575	3362800..3363276	+	477	WP_001186726.1	RadC family protein	-
I14_RS16580	3363339..3363560	+	222	WP_000692329.1	DUF987 domain-containing protein	-
I14_RS16585	3363579..3364223	+	645	WP_000094916.1	hypothetical protein	-
I14_RS16590	3364273..3364641	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I14_RS16595	3364730..3365107	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
I14_RS16600	3365104..3365592	+	489	WP_000761699.1	hypothetical protein	-
I14_RS16605	3365609..3365806	+	198	WP_000839293.1	DUF957 domain-containing protein	-
I14_RS16610	3365891..3366736	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
I14_RS16615	3366805..3367200	+	396	WP_000208382.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
I14_RS16620	3367193..3368126	+	934	Protein_3140	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
I14_RS28265	3368558..3368713	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / ugd / kpsT	3334443..3386316	51873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T27554 WP_000854690.1 NC_017652:3364730-3365107 [Escherichia coli str. 'clone D i14']
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T27554 NC_017652:3364730-3365107 [Escherichia coli str. 'clone D i14']
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT27554 WP_001305076.1 NC_017652:3364273-3364641 [Escherichia coli str. 'clone D i14']
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT27554 NC_017652:3364273-3364641 [Escherichia coli str. 'clone D i14']
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8