275486

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 920028..920721 Replicon chromosome
Accession NZ_CP120969
Organism Pseudomonas putida strain AHSWHJXPP1

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag P3X84_RS04330 Protein ID WP_003151133.1
Coordinates 920028..920405 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag P3X84_RS04335 Protein ID WP_001172026.1
Coordinates 920386..920721 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P3X84_RS04320 (P3X84_04320) 916221..919250 - 3030 WP_010799689.1 Tn3 family transposase -
P3X84_RS04325 (P3X84_04325) 919234..919836 - 603 WP_010465829.1 recombinase family protein -
P3X84_RS04330 (P3X84_04330) 920028..920405 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P3X84_RS04335 (P3X84_04335) 920386..920721 + 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
P3X84_RS04340 (P3X84_04340) 920736..921071 + 336 WP_000741275.1 hypothetical protein -
P3X84_RS04345 (P3X84_04345) 921095..921316 + 222 Protein_834 hypothetical protein -
P3X84_RS04350 (P3X84_04350) 922004..923521 - 1518 WP_277836186.1 IS66 family transposase -
P3X84_RS04355 (P3X84_04355) 923540..923899 - 360 WP_024086151.1 IS66 family insertion sequence element accessory protein TnpB -
P3X84_RS04360 (P3X84_04360) 924057..925445 - 1389 Protein_837 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island aph(3'')-Ib / blaNDM-14 / sul1 / qacE / blaOXA-427 / qnrVC6 / mph(E) / msr(E) / ant(3'')-Ia / catB3 / ere(A) - 877529..954476 76947
- inside IScluster/Tn blaOXA-427 / qnrVC6 / mph(E) / msr(E) / sul1 / qacE / ant(3'')-Ia / catB3 / ere(A) - 902200..945555 43355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T275486 WP_003151133.1 NZ_CP120969:920028-920405 [Pseudomonas putida]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT275486 WP_001172026.1 NZ_CP120969:920386-920721 [Pseudomonas putida]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References