Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 242929..243571 Replicon chromosome
Accession NZ_CP120958
Organism Bacillus cereus strain SCBCM001

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag P4G50_RS01385 Protein ID WP_000635963.1
Coordinates 243221..243571 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag P4G50_RS01380 Protein ID WP_000004570.1
Coordinates 242929..243216 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P4G50_RS01355 238246..239208 + 963 WP_000961165.1 UV DNA damage repair endonuclease UvsE -
P4G50_RS01360 239201..239773 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
P4G50_RS01365 239867..240226 + 360 WP_000583416.1 holo-ACP synthase -
P4G50_RS01370 240383..241333 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
P4G50_RS01375 241451..242620 + 1170 WP_000390615.1 alanine racemase -
P4G50_RS01380 242929..243216 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
P4G50_RS01385 243221..243571 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P4G50_RS01390 243639..245807 + 2169 WP_000426242.1 Tex family protein -
P4G50_RS01395 245865..245981 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
P4G50_RS01400 246177..246635 + 459 WP_000344234.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T275483 WP_000635963.1 NZ_CP120958:243221-243571 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT275483 WP_000004570.1 NZ_CP120958:242929-243216 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References