Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/HTH_IclR(antitoxin)
Location 1222997..1224747 Replicon chromosome
Accession NZ_CP120948
Organism Burkholderia contaminans strain 5080

Toxin (Protein)


Gene name doc Uniprot ID A0A1E3FSX8
Locus tag P4E65_RS23040 Protein ID WP_039344096.1
Coordinates 1222997..1223893 (+) Length 299 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A1E3FSY5
Locus tag P4E65_RS23045 Protein ID WP_039344097.1
Coordinates 1223890..1224747 (+) Length 286 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P4E65_RS23010 (P4E65_23010) 1218367..1219446 - 1080 WP_039344086.1 porin -
P4E65_RS23015 (P4E65_23015) 1219891..1220208 + 318 WP_011354710.1 non-heme iron oxygenase ferredoxin subunit -
P4E65_RS23020 (P4E65_23020) 1220210..1220521 + 312 WP_039344088.1 hypothetical protein -
P4E65_RS23025 (P4E65_23025) 1220574..1221650 + 1077 WP_039344090.1 aromatic ring-hydroxylating dioxygenase subunit alpha -
P4E65_RS23030 (P4E65_23030) 1221643..1222413 + 771 WP_039344092.1 SDR family oxidoreductase -
P4E65_RS23035 (P4E65_23035) 1222451..1222978 + 528 WP_039344094.1 cupin domain-containing protein -
P4E65_RS23040 (P4E65_23040) 1222997..1223893 + 897 WP_039344096.1 alpha/beta fold hydrolase Toxin
P4E65_RS23045 (P4E65_23045) 1223890..1224747 + 858 WP_039344097.1 IclR family transcriptional regulator Antitoxin
P4E65_RS23050 (P4E65_23050) 1224744..1225541 + 798 WP_039344099.1 SDR family oxidoreductase -
P4E65_RS23055 (P4E65_23055) 1225534..1227201 + 1668 WP_039344101.1 thiamine pyrophosphate-binding protein -
P4E65_RS23060 (P4E65_23060) 1227194..1228021 + 828 WP_039344103.1 aspartate dehydrogenase -
P4E65_RS23065 (P4E65_23065) 1228094..1229584 + 1491 WP_039344105.1 aldehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Alpha/beta hydrolase fold-1

(51-279)

Antitoxin

Transcription regulator IclR, C-terminal

(143-266)


Sequences

Toxin        


Download         Length: 299 a.a.        Molecular weight: 30700.99 Da        Isoelectric Point: 8.0624

>T275470 WP_039344096.1 NZ_CP120948:1222997-1223893 [Burkholderia contaminans]
MSVIDKRERDRTAAFAALLGQFPEQRCAAGAAGTIGYREAGAHHAGRALPVVLLHGIGSGAASWVRQLDTLGASRRVLAW
DAPGYGVSTPVHGASPAATDYAASLNAWLEALGIERCVLVGHSLGAIIAGGLIRVMPARIAGLLLVSPAGGYGSAPAETR
DARRDARLAMLAELGPAGLAEQRSGNMLSGFANEDARAWVRWNMARIVPAGYAQATQLLANADLATDLAGFRGRTAVAVG
ANDAITPPAACERIAAAAHVGLQVIPQAGHAGYVEAPAVYSALIDAFCRQCDQQRGLA

Download         Length: 897 bp

Antitoxin


Download         Length: 286 a.a.        Molecular weight: 31463.03 Da        Isoelectric Point: 6.7661

>AT275470 WP_039344097.1 NZ_CP120948:1223890-1224747 [Burkholderia contaminans]
MNEPLQRPELENGKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYK
LGIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSQAPVFSSIRVNVGTRLPAHATTHGH
VLMGDLSLKELHALYPEGTLNRMTNATPETVDALYEVIREDALRGYGVSNSSFERGISVVTAPVRNETQRIVACITVTVP
RPEIDAALIADGLIDKVQRAAAELSRRLNYRSDDEHTFLKALGLR

Download         Length: 858 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1E3FSX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1E3FSY5

References