Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4955717..4956214 Replicon chromosome
Accession NZ_CP120944
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag P7249_RS24820 Protein ID WP_001173343.1
Coordinates 4955717..4955890 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag P7249_RS24825 Protein ID WP_000398619.1
Coordinates 4955918..4956214 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P7249_RS24810 (4952387) 4952387..4953844 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
P7249_RS24815 (4954081) 4954081..4955598 + 1518 WP_001295074.1 lysine--tRNA ligase -
P7249_RS24820 (4955717) 4955717..4955890 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
P7249_RS24825 (4955918) 4955918..4956214 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
P7249_RS24830 (4956441) 4956441..4956713 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
P7249_RS24835 (4956725) 4956725..4956955 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
P7249_RS24840 (4957136) 4957136..4958767 + 1632 WP_001216477.1 sensor histidine kinase -
P7249_RS24845 (4958764) 4958764..4959483 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T275464 WP_001173343.1 NZ_CP120944:c4955890-4955717 [Escherichia coli O157:H7 str. EDL933]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT275464 WP_000398619.1 NZ_CP120944:c4956214-4955918 [Escherichia coli O157:H7 str. EDL933]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References