275441

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 352311..353111 Replicon chromosome
Accession NZ_CP120944
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name ataT Uniprot ID Q8XED1
Locus tag P7249_RS01710 Protein ID WP_000342450.1
Coordinates 352584..353111 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag P7249_RS01705 Protein ID WP_001277108.1
Coordinates 352311..352577 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P7249_RS01685 (347969) 347969..348637 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
P7249_RS01690 (348630) 348630..349688 + 1059 WP_001042018.1 permease-like cell division protein FtsX -
P7249_RS01695 (349933) 349933..350787 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
P7249_RS01700 (351058) 351058..352161 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
P7249_RS01705 (352311) 352311..352577 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
P7249_RS01710 (352584) 352584..353111 + 528 WP_000342450.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
P7249_RS01715 (353108) 353108..353491 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
P7249_RS01720 (353915) 353915..355024 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
P7249_RS01725 (355072) 355072..355998 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
P7249_RS01730 (355995) 355995..357272 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
P7249_RS01735 (357269) 357269..358036 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19678.61 Da        Isoelectric Point: 6.9489

>T275441 WP_000342450.1 NZ_CP120944:352584-353111 [Escherichia coli O157:H7 str. EDL933]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMNVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT275441 WP_001277108.1 NZ_CP120944:352311-352577 [Escherichia coli O157:H7 str. EDL933]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7CHD

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References