Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5373621..5374246 Replicon chromosome
Accession NZ_CP120847
Organism Klebsiella pneumoniae strain KPHRJ

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag P5D18_RS26045 Protein ID WP_002882817.1
Coordinates 5373621..5374004 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag P5D18_RS26050 Protein ID WP_004150355.1
Coordinates 5374004..5374246 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5D18_RS26030 (5370987) 5370987..5371889 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
P5D18_RS26035 (5371886) 5371886..5372521 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
P5D18_RS26040 (5372518) 5372518..5373447 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
P5D18_RS26045 (5373621) 5373621..5374004 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
P5D18_RS26050 (5374004) 5374004..5374246 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
P5D18_RS26055 (5374451) 5374451..5375368 + 918 WP_004181612.1 alpha/beta hydrolase -
P5D18_RS26060 (5375382) 5375382..5376323 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
P5D18_RS26065 (5376368) 5376368..5376805 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
P5D18_RS26070 (5376802) 5376802..5377662 - 861 WP_004146232.1 virulence factor BrkB family protein -
P5D18_RS26075 (5377656) 5377656..5378255 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T275428 WP_002882817.1 NZ_CP120847:c5374004-5373621 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT275428 WP_004150355.1 NZ_CP120847:c5374246-5374004 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References