Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 532933..533569 Replicon chromosome
Accession NZ_CP120846
Organism Bacillus atrophaeus strain MBLB1156

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P4829_RS02655 Protein ID WP_003156187.1
Coordinates 533219..533569 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A837XXL9
Locus tag P4829_RS02650 Protein ID WP_003328141.1
Coordinates 532933..533214 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P4829_RS02630 (529229) 529229..529828 - 600 WP_010789631.1 rhomboid family intramembrane serine protease -
P4829_RS02635 (529928) 529928..530293 + 366 WP_061669621.1 holo-ACP synthase -
P4829_RS02640 (530461) 530461..531477 + 1017 WP_094231401.1 outer membrane lipoprotein carrier protein LolA -
P4829_RS02645 (531645) 531645..532814 + 1170 WP_063638785.1 alanine racemase -
P4829_RS02650 (532933) 532933..533214 + 282 WP_003328141.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P4829_RS02655 (533219) 533219..533569 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P4829_RS02660 (533685) 533685..534503 + 819 WP_010789627.1 RsbT co-antagonist protein RsbRA -
P4829_RS02665 (534508) 534508..534873 + 366 WP_010789626.1 RsbT antagonist protein RsbS -
P4829_RS02670 (534876) 534876..535277 + 402 WP_003328144.1 serine/threonine-protein kinase RsbT -
P4829_RS02675 (535289) 535289..536296 + 1008 WP_063638786.1 PP2C family protein-serine/threonine phosphatase -
P4829_RS02680 (536356) 536356..536685 + 330 WP_003328146.1 anti-sigma factor antagonist RsbV -
P4829_RS02685 (536682) 536682..537164 + 483 WP_003328147.1 anti-sigma B factor RsbW -
P4829_RS02690 (537130) 537130..537918 + 789 WP_003328149.1 RNA polymerase sigma factor SigB -
P4829_RS02695 (537918) 537918..538517 + 600 WP_003328150.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T275413 WP_003156187.1 NZ_CP120846:533219-533569 [Bacillus atrophaeus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10451.83 Da        Isoelectric Point: 5.1903

>AT275413 WP_003328141.1 NZ_CP120846:532933-533214 [Bacillus atrophaeus]
MSESSATTEMMISLPEALVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB A0A837XXL9

References