Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 586649..587285 | Replicon | chromosome |
| Accession | NZ_CP120723 | ||
| Organism | Bacillus atrophaeus strain NX-12 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | P3T49_RS03025 | Protein ID | WP_003156187.1 |
| Coordinates | 586935..587285 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A837XXL9 |
| Locus tag | P3T49_RS03020 | Protein ID | WP_003328141.1 |
| Coordinates | 586649..586930 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| P3T49_RS03000 (582946) | 582946..583545 | - | 600 | WP_277714795.1 | rhomboid family intramembrane serine protease | - |
| P3T49_RS03005 (583645) | 583645..584010 | + | 366 | WP_061669621.1 | holo-ACP synthase | - |
| P3T49_RS03010 (584178) | 584178..585194 | + | 1017 | WP_277714346.1 | outer membrane lipoprotein carrier protein LolA | - |
| P3T49_RS03015 (585361) | 585361..586530 | + | 1170 | WP_277714347.1 | alanine racemase | - |
| P3T49_RS03020 (586649) | 586649..586930 | + | 282 | WP_003328141.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| P3T49_RS03025 (586935) | 586935..587285 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| P3T49_RS03030 (587401) | 587401..588219 | + | 819 | WP_106272957.1 | RsbT co-antagonist protein RsbRA | - |
| P3T49_RS03035 (588224) | 588224..588589 | + | 366 | WP_010789626.1 | RsbT antagonist protein RsbS | - |
| P3T49_RS03040 (588592) | 588592..588993 | + | 402 | WP_003328144.1 | serine/threonine-protein kinase RsbT | - |
| P3T49_RS03045 (589005) | 589005..590012 | + | 1008 | WP_151291230.1 | PP2C family protein-serine/threonine phosphatase | - |
| P3T49_RS03050 (590072) | 590072..590401 | + | 330 | WP_106360768.1 | anti-sigma factor antagonist RsbV | - |
| P3T49_RS03055 (590398) | 590398..590880 | + | 483 | WP_003328147.1 | anti-sigma B factor RsbW | - |
| P3T49_RS03060 (590846) | 590846..591634 | + | 789 | WP_106047471.1 | RNA polymerase sigma factor SigB | - |
| P3T49_RS03065 (591634) | 591634..592233 | + | 600 | WP_003328150.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T275380 WP_003156187.1 NZ_CP120723:586935-587285 [Bacillus atrophaeus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T275380 NZ_CP120723:586935-587285 [Bacillus atrophaeus]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGAGTGCGCCCGGT
TTTGGTGATTCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATTTTGCTGGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATTAGTTTGGCACTCATTGATTTTTAG
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGAGTGCGCCCGGT
TTTGGTGATTCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATTTTGCTGGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATTAGTTTGGCACTCATTGATTTTTAG
Antitoxin
Download Length: 94 a.a. Molecular weight: 10451.83 Da Isoelectric Point: 5.1903
>AT275380 WP_003328141.1 NZ_CP120723:586649-586930 [Bacillus atrophaeus]
MSESSATTEMMISLPEALVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSATTEMMISLPEALVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT275380 NZ_CP120723:586649-586930 [Bacillus atrophaeus]
TTGTCTGAATCCAGCGCAACAACCGAAATGATGATTAGCTTGCCGGAAGCACTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTAATATCACAAGCAGTGAGAGCCTATGTCAGCGAACGCACAACTCGTCACAACC
GTGATTTGATGAGACGCGGATATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAAGCTCATTTTGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA
TTGTCTGAATCCAGCGCAACAACCGAAATGATGATTAGCTTGCCGGAAGCACTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTAATATCACAAGCAGTGAGAGCCTATGTCAGCGAACGCACAACTCGTCACAACC
GTGATTTGATGAGACGCGGATATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAAGCTCATTTTGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| PDB | 4MDX | |
| PDB | 4ME7 | |
| PDB | 1NE8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A837XXL9 |