Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 555554..556172 Replicon chromosome
Accession NC_017652
Organism Escherichia coli str. clone D i14

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I14_RS02680 Protein ID WP_001291435.1
Coordinates 555554..555772 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I14_RS02685 Protein ID WP_000344800.1
Coordinates 555798..556172 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I14_RS02650 550842..551414 + 573 WP_000779831.1 YbaY family lipoprotein -
I14_RS02655 551445..551756 - 312 WP_000409908.1 MGMT family protein -
I14_RS02660 552135..552488 + 354 WP_000878144.1 DUF1428 family protein -
I14_RS02665 552530..554080 - 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I14_RS02670 554244..554714 - 471 WP_001304825.1 YlaC family protein -
I14_RS02675 554830..555381 - 552 WP_000102539.1 maltose O-acetyltransferase -
I14_RS02680 555554..555772 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I14_RS02685 555798..556172 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I14_RS02690 556717..559866 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
I14_RS02695 559889..561082 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T27538 WP_001291435.1 NC_017652:c555772-555554 [Escherichia coli str. 'clone D i14']
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T27538 NC_017652:c555772-555554 [Escherichia coli str. 'clone D i14']
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT27538 WP_000344800.1 NC_017652:c556172-555798 [Escherichia coli str. 'clone D i14']
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT27538 NC_017652:c556172-555798 [Escherichia coli str. 'clone D i14']
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References