Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3033528..3034165 Replicon chromosome
Accession NZ_CP120711
Organism Bacillus velezensis strain 12Y

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P0M29_RS15455 Protein ID WP_003156187.1
Coordinates 3033815..3034165 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag P0M29_RS15450 Protein ID WP_003156188.1
Coordinates 3033528..3033809 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P0M29_RS15430 (3029893) 3029893..3030492 - 600 WP_029974099.1 rhomboid family intramembrane serine protease -
P0M29_RS15435 (3030585) 3030585..3030950 + 366 WP_007609580.1 holo-ACP synthase -
P0M29_RS15440 (3031115) 3031115..3032122 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
P0M29_RS15445 (3032239) 3032239..3033408 + 1170 WP_021495079.1 alanine racemase -
P0M29_RS15450 (3033528) 3033528..3033809 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P0M29_RS15455 (3033815) 3033815..3034165 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P0M29_RS15460 (3034283) 3034283..3035104 + 822 WP_003156182.1 STAS domain-containing protein -
P0M29_RS15465 (3035109) 3035109..3035474 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
P0M29_RS15470 (3035477) 3035477..3035878 + 402 WP_003156178.1 anti-sigma regulatory factor -
P0M29_RS15475 (3035890) 3035890..3036897 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
P0M29_RS15480 (3036961) 3036961..3037290 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
P0M29_RS15485 (3037287) 3037287..3037769 + 483 WP_007609591.1 anti-sigma B factor RsbW -
P0M29_RS15490 (3037735) 3037735..3038523 + 789 WP_029974100.1 RNA polymerase sigma factor SigB -
P0M29_RS15495 (3038523) 3038523..3039125 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T275374 WP_003156187.1 NZ_CP120711:3033815-3034165 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT275374 WP_003156188.1 NZ_CP120711:3033528-3033809 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References