Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2625922..2626573 Replicon chromosome
Accession NZ_CP120688
Organism Lacticaseibacillus sp. DSM 115424

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag LHUE2_RS12185 Protein ID WP_005686631.1
Coordinates 2625922..2626305 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0R1EMB2
Locus tag LHUE2_RS12190 Protein ID WP_010491246.1
Coordinates 2626325..2626573 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHUE2_RS12165 (LHUE2_002433) 2621401..2622408 + 1008 WP_003662706.1 L-lactate dehydrogenase -
LHUE2_RS12170 (LHUE2_002434) 2622715..2624085 + 1371 WP_049170368.1 L,D-transpeptidase family protein -
LHUE2_RS12175 (LHUE2_002435) 2624293..2624958 + 666 WP_010491258.1 cyclic di-AMP binding protein CbpA -
LHUE2_RS12180 (LHUE2_002436) 2625154..2625852 + 699 WP_049170367.1 L,D-transpeptidase -
LHUE2_RS12185 (LHUE2_002437) 2625922..2626305 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LHUE2_RS12190 (LHUE2_002438) 2626325..2626573 - 249 WP_010491246.1 antitoxin Antitoxin
LHUE2_RS12195 (LHUE2_002439) 2626672..2627811 - 1140 WP_049170365.1 alanine racemase -
LHUE2_RS12200 (LHUE2_002440) 2627798..2628172 - 375 WP_049170363.1 holo-ACP synthase -
LHUE2_RS12205 (LHUE2_002441) 2628419..2629930 - 1512 WP_049170360.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T275372 WP_005686631.1 NZ_CP120688:c2626305-2625922 [Lacticaseibacillus sp. DSM 115424]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8971.07 Da        Isoelectric Point: 4.6061

>AT275372 WP_010491246.1 NZ_CP120688:c2626573-2626325 [Lacticaseibacillus sp. DSM 115424]
MKKQTSVTVTFDAAIKARLDAYCELNDTDTQDVITTAVAKFLQSQAPDINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB A0A0R1EMB2

References