Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 4099037..4099673 Replicon chromosome
Accession NZ_CP120681
Organism Bacillus subtilis strain DSM 10

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P5655_RS21925 Protein ID WP_003156187.1
Coordinates 4099323..4099673 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag P5655_RS21920 Protein ID WP_003225183.1
Coordinates 4099037..4099318 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5655_RS21900 (4095396) 4095396..4095995 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
P5655_RS21905 (4096090) 4096090..4096455 + 366 WP_003234281.1 holo-ACP synthase -
P5655_RS21910 (4096621) 4096621..4097637 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
P5655_RS21915 (4097752) 4097752..4098921 + 1170 WP_003234284.1 alanine racemase -
P5655_RS21920 (4099037) 4099037..4099318 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P5655_RS21925 (4099323) 4099323..4099673 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P5655_RS21930 (4099788) 4099788..4100612 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
P5655_RS21935 (4100617) 4100617..4100982 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
P5655_RS21940 (4100986) 4100986..4101387 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
P5655_RS21945 (4101399) 4101399..4102406 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
P5655_RS21950 (4102468) 4102468..4102797 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
P5655_RS21955 (4102794) 4102794..4103276 + 483 WP_003234299.1 anti-sigma B factor RsbW -
P5655_RS21960 (4103242) 4103242..4104030 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
P5655_RS21965 (4104030) 4104030..4104629 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T275369 WP_003156187.1 NZ_CP120681:4099323-4099673 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT275369 WP_003225183.1 NZ_CP120681:4099037-4099318 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References