275349

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 713161..714077 Replicon chromosome
Accession NZ_CP120681
Organism Bacillus subtilis strain DSM 10

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag P5655_RS04240 Protein ID WP_003244695.1
Coordinates 713331..714077 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag P5655_RS04235 Protein ID WP_003232646.1
Coordinates 713161..713331 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5655_RS04200 (710024) 710024..710353 + 330 WP_003232660.1 XkdW family protein -
P5655_RS04205 (710350) 710350..710514 + 165 WP_003232658.1 XkdX family protein -
P5655_RS04210 (710558) 710558..711397 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
P5655_RS04215 (711450) 711450..711719 + 270 WP_003232655.1 hemolysin XhlA family protein -
P5655_RS04220 (711732) 711732..711995 + 264 WP_003232653.1 phage holin -
P5655_RS04225 (712008) 712008..712901 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
P5655_RS04230 (712938) 712938..713075 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
P5655_RS04235 (713161) 713161..713331 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
P5655_RS04240 (713331) 713331..714077 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
P5655_RS04245 (714187) 714187..715188 - 1002 WP_003232642.1 inorganic phosphate transporter -
P5655_RS04250 (715201) 715201..715818 - 618 WP_003218470.1 DUF47 domain-containing protein -
P5655_RS04255 (716094) 716094..717410 - 1317 WP_003244819.1 serine/threonine exchanger -
P5655_RS04260 (717799) 717799..718749 + 951 WP_003245086.1 ring-cleaving dioxygenase -
P5655_RS04265 (718850) 718850..718996 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T275349 WP_003244695.1 NZ_CP120681:c714077-713331 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT275349 WP_003232646.1 NZ_CP120681:c713331-713161 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References