Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 489965..490602 Replicon chromosome
Accession NZ_CP120637
Organism Bacillus velezensis strain LQ-3

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P3M44_RS02505 Protein ID WP_003156187.1
Coordinates 490252..490602 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag P3M44_RS02500 Protein ID WP_003156188.1
Coordinates 489965..490246 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P3M44_RS02480 (P3M44_02480) 486330..486929 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
P3M44_RS02485 (P3M44_02485) 487022..487387 + 366 WP_007609580.1 holo-ACP synthase -
P3M44_RS02490 (P3M44_02490) 487552..488559 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
P3M44_RS02495 (P3M44_02495) 488676..489845 + 1170 WP_032873001.1 alanine racemase -
P3M44_RS02500 (P3M44_02500) 489965..490246 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P3M44_RS02505 (P3M44_02505) 490252..490602 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P3M44_RS02510 (P3M44_02510) 490720..491541 + 822 WP_003156182.1 STAS domain-containing protein -
P3M44_RS02515 (P3M44_02515) 491546..491911 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
P3M44_RS02520 (P3M44_02520) 491914..492315 + 402 WP_003156178.1 anti-sigma regulatory factor -
P3M44_RS02525 (P3M44_02525) 492327..493334 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
P3M44_RS02530 (P3M44_02530) 493398..493727 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
P3M44_RS02535 (P3M44_02535) 493724..494206 + 483 WP_007609591.1 anti-sigma B factor RsbW -
P3M44_RS02540 (P3M44_02540) 494172..494960 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
P3M44_RS02545 (P3M44_02545) 494960..495562 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T275312 WP_003156187.1 NZ_CP120637:490252-490602 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT275312 WP_003156188.1 NZ_CP120637:489965-490246 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References