Detailed information of TA system TA275308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4417109..4418798
• Replicon: chromosome
• Accession: NZ_CP120633
• Organism: Escherichia coli strain USVAST219

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: P6O79_RS21620
• Protein ID: WP_000365807.1
• Coordinates: 4417878..4418798 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: P6O79_RS21615
• Protein ID: WP_001295795.1
• Coordinates: 4417109..4417891 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P6O79_RS21610 (4413727)	4413727..4416825	-	3099	WP_000673070.1	type I restriction endonuclease	-
P6O79_RS21615 (4417109)	4417109..4417891	+	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
P6O79_RS21620 (4417878)	4417878..4418798	+	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
P6O79_RS21625 (4418963)	4418963..4419739	-	777	WP_000171155.1	site-specific integrase	-
P6O79_RS21630 (4419947)	4419947..4420282	-	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
P6O79_RS21635 (4420509)	4420509..4420667	-	159	Protein_4228	integrase	-
P6O79_RS21640 (4420716)	4420716..4420901	+	186	WP_000146240.1	hypothetical protein	-
P6O79_RS21645 (4421116)	4421116..4421730	+	615	WP_001019923.1	YagU family protein	-
P6O79_RS21650 (4421801)	4421801..4421967	+	167	Protein_4231	tail fiber assembly protein	-
P6O79_RS21655 (4421979)	4421979..4422308	-	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
P6O79_RS21660 (4422615)	4422615..4423325	-	711	WP_001295798.1	fimbrial chaperone EcpE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	4410859..4459014	48155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T275308 WP_000365807.1 NZ_CP120633:4417878-4418798 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT275308 WP_001295795.1 NZ_CP120633:4417109-4417891 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0