Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2239630..2240284 Replicon chromosome
Accession NZ_CP120633
Organism Escherichia coli strain USVAST219

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag P6O79_RS11215 Protein ID WP_000244781.1
Coordinates 2239630..2240037 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag P6O79_RS11220 Protein ID WP_000354046.1
Coordinates 2240018..2240284 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6O79_RS11195 (2235587) 2235587..2237320 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
P6O79_RS11200 (2237326) 2237326..2238036 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
P6O79_RS11205 (2238061) 2238061..2238957 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
P6O79_RS11210 (2239069) 2239069..2239590 + 522 WP_001055874.1 flavodoxin FldB -
P6O79_RS11215 (2239630) 2239630..2240037 - 408 WP_000244781.1 protein YgfX Toxin
P6O79_RS11220 (2240018) 2240018..2240284 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
P6O79_RS11225 (2240527) 2240527..2241507 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
P6O79_RS11230 (2241584) 2241584..2242243 - 660 WP_000250274.1 hemolysin III family protein -
P6O79_RS11235 (2242407) 2242407..2242718 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
P6O79_RS11240 (2242763) 2242763..2244196 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T275297 WP_000244781.1 NZ_CP120633:c2240037-2239630 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT275297 WP_000354046.1 NZ_CP120633:c2240284-2240018 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References