Detailed information of TA system TA027529

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4921076..4921902
• Replicon: chromosome
• Accession: NC_017651
• Organism: Escherichia coli str. clone D i2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: I02_RS24170
• Protein ID: WP_000854683.1
• Coordinates: 4921528..4921902 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I02_RS24165
• Protein ID: WP_024186744.1
• Coordinates: 4921076..4921438 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I02_RS24130	4916508..4917413	+	906	WP_000203561.1	chemotaxis protein	-
I02_RS24135	4917410..4918480	+	1071	WP_000102675.1	patatin-like phospholipase family protein	-
I02_RS24145	4918820..4919638	+	819	WP_001234592.1	DUF945 domain-containing protein	-
I02_RS24150	4919730..4920215	+	486	WP_000206672.1	antirestriction protein	-
I02_RS24155	4920231..4920707	+	477	WP_001186708.1	RadC family protein	-
I02_RS24160	4920776..4920997	+	222	WP_000692312.1	DUF987 domain-containing protein	-
I02_RS24165	4921076..4921438	+	363	WP_024186744.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I02_RS24170	4921528..4921902	+	375	WP_000854683.1	TA system toxin CbtA family protein	Toxin
I02_RS24175	4921899..4922390	+	492	WP_000976831.1	hypothetical protein	-
I02_RS24180	4922402..4922599	+	198	WP_000839235.1	DUF957 domain-containing protein	-
I02_RS28300	4922684..4922836	+	153	WP_001555250.1	hypothetical protein	-
I02_RS24185	4923300..4924373	+	1074	WP_001327207.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (16-115)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13993.03 Da
• Isoelectric Point: 8.2905

>T27529 WP_000854683.1 NC_017651:4921528-4921902 [Escherichia coli str. 'clone D i2']
MKTLPDTHIREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTQFADERVIELHIEAGISLCDAVNFLVEKYALVRTGQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T27529 NC_017651:4921528-4921902 [Escherichia coli str. 'clone D i2']
ATGAAAACATTACCCGACACACACATACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGACCAACATTACGGCCTTACGCTGAATGACACACAGTTCGCGGATGAACGCGTGATTGAGCTGCATATTG
AGGCCGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGGCCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13354.17 Da
• Isoelectric Point: 6.6247

>AT27529 WP_024186744.1 NC_017651:4921076-4921438 [Escherichia coli str. 'clone D i2']
VSDTLPGTTHPDNNRPWWGLPCTVMPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGEL
NPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 363 bp

>AT27529 NC_017651:4921076-4921438 [Escherichia coli str. 'clone D i2']
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACCGCCCCTGGTGGGGGCTACCCTGCACCGTGATGCCCTG
TTTCGGCGCCCGTCTGGTGCAGGAAGGCAATCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGTTCAGCG
ACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTG
AATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGATACCCTCGGCAGTTGTGGTTA
CGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAAAGTAA