Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 488468..489104 Replicon chromosome
Accession NZ_CP120622
Organism Bacillus subtilis strain DSM 1090

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P5647_RS02490 Protein ID WP_003156187.1
Coordinates 488754..489104 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag P5647_RS02485 Protein ID WP_003225183.1
Coordinates 488468..488749 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5647_RS02465 (484827) 484827..485426 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
P5647_RS02470 (485521) 485521..485886 + 366 WP_003234281.1 holo-ACP synthase -
P5647_RS02475 (486052) 486052..487068 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
P5647_RS02480 (487183) 487183..488352 + 1170 WP_003234284.1 alanine racemase -
P5647_RS02485 (488468) 488468..488749 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P5647_RS02490 (488754) 488754..489104 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P5647_RS02495 (489219) 489219..490043 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
P5647_RS02500 (490048) 490048..490413 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
P5647_RS02505 (490417) 490417..490818 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
P5647_RS02510 (490830) 490830..491837 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
P5647_RS02515 (491899) 491899..492228 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
P5647_RS02520 (492225) 492225..492707 + 483 WP_003234299.1 anti-sigma B factor RsbW -
P5647_RS02525 (492673) 492673..493461 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
P5647_RS02530 (493461) 493461..494060 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T275275 WP_003156187.1 NZ_CP120622:488754-489104 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT275275 WP_003225183.1 NZ_CP120622:488468-488749 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References