Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 3314517..3315433 Replicon chromosome
Accession NZ_CP120621
Organism Bacillus subtilis strain DSM 13019

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag P5657_RS17470 Protein ID WP_003244695.1
Coordinates 3314517..3315263 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag P5657_RS17475 Protein ID WP_003232646.1
Coordinates 3315263..3315433 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5657_RS17445 (P5657_17445) 3309635..3309736 - 102 Protein_3391 hypothetical protein -
P5657_RS17450 (P5657_17450) 3309845..3310795 - 951 WP_277710026.1 ring-cleaving dioxygenase -
P5657_RS17455 (P5657_17455) 3311184..3312500 + 1317 WP_021479457.1 serine/threonine exchanger -
P5657_RS17460 (P5657_17460) 3312776..3313393 + 618 WP_003218470.1 DUF47 domain-containing protein -
P5657_RS17465 (P5657_17465) 3313406..3314407 + 1002 WP_021479458.1 inorganic phosphate transporter -
P5657_RS17470 (P5657_17470) 3314517..3315263 + 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
P5657_RS17475 (P5657_17475) 3315263..3315433 + 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
P5657_RS17480 (P5657_17480) 3315519..3315656 + 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
P5657_RS17485 (P5657_17485) 3315693..3316586 - 894 WP_021479459.1 N-acetylmuramoyl-L-alanine amidase -
P5657_RS17490 (P5657_17490) 3316599..3316862 - 264 WP_014479566.1 phage holin -
P5657_RS17495 (P5657_17495) 3316875..3317144 - 270 WP_014479565.1 hemolysin XhlA family protein -
P5657_RS17500 (P5657_17500) 3317197..3318036 - 840 WP_163182364.1 phage-like element PBSX protein XepA -
P5657_RS17505 (P5657_17505) 3318083..3318247 - 165 WP_014479563.1 XkdX family protein -
P5657_RS17510 (P5657_17510) 3318244..3318573 - 330 WP_014479562.1 XkdW family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T275274 WP_003244695.1 NZ_CP120621:3314517-3315263 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT275274 WP_003232646.1 NZ_CP120621:3315263-3315433 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References