Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 93994..94630 Replicon chromosome
Accession NZ_CP120621
Organism Bacillus subtilis strain DSM 13019

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P5657_RS00560 Protein ID WP_003156187.1
Coordinates 93994..94344 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag P5657_RS00565 Protein ID WP_003225183.1
Coordinates 94349..94630 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5657_RS00520 (P5657_00520) 89030..89629 - 600 WP_021481701.1 phosphoserine phosphatase RsbX -
P5657_RS00525 (P5657_00525) 89629..90417 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
P5657_RS00530 (P5657_00530) 90383..90865 - 483 WP_021481702.1 anti-sigma B factor RsbW -
P5657_RS00535 (P5657_00535) 90862..91191 - 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
P5657_RS00540 (P5657_00540) 91260..92267 - 1008 WP_134991884.1 phosphoserine phosphatase RsbU -
P5657_RS00545 (P5657_00545) 92279..92680 - 402 WP_003225192.1 serine/threonine-protein kinase RsbT -
P5657_RS00550 (P5657_00550) 92684..93049 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
P5657_RS00555 (P5657_00555) 93054..93878 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
P5657_RS00560 (P5657_00560) 93994..94344 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P5657_RS00565 (P5657_00565) 94349..94630 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P5657_RS00570 (P5657_00570) 94746..95915 - 1170 WP_277710154.1 alanine racemase -
P5657_RS00575 (P5657_00575) 96029..97045 - 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
P5657_RS00580 (P5657_00580) 97211..97576 - 366 WP_015252768.1 holo-ACP synthase -
P5657_RS00585 (P5657_00585) 97671..98270 + 600 WP_003246687.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T275273 WP_003156187.1 NZ_CP120621:c94344-93994 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT275273 WP_003225183.1 NZ_CP120621:c94630-94349 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References