Detailed information of TA system TA027526

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4159684..4160475
• Replicon: chromosome
• Accession: NC_017651
• Organism: Escherichia coli str. clone D i2

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: I02_RS20510
• Protein ID: WP_000691791.1
• Coordinates: 4160050..4160475 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: I02_RS20505
• Protein ID: WP_000939437.1
• Coordinates: 4159684..4160019 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I02_RS20480	4155635..4155853	-	219	WP_000070772.1	AlpA family phage regulatory protein	-
I02_RS20485	4155971..4156585	-	615	WP_000772910.1	inovirus Gp2 family protein	-
I02_RS20490	4157178..4158131	+	954	WP_000290406.1	hypothetical protein	-
I02_RS20495	4158367..4159181	+	815	Protein_3883	DUF945 domain-containing protein	-
I02_RS20500	4159211..4159684	+	474	WP_001292742.1	DNA repair protein RadC	-
I02_RS20505	4159684..4160019	+	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I02_RS20510	4160050..4160475	+	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
I02_RS20515	4160496..4161687	+	1192	Protein_3887	AAA family ATPase	-
I02_RS28010	4161672..4161893	+	222	WP_125112077.1	hypothetical protein	-
I02_RS20520	4161890..4162621	+	732	WP_001216639.1	TIGR02646 family protein	-
I02_RS20530	4163244..4163630	+	387	WP_001270938.1	DUF2441 domain-containing protein	-
I02_RS20540	4164312..4164809	+	498	WP_000509808.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4148806..4164809	16003

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T27526 WP_000691791.1 NC_017651:4160050-4160475 [Escherichia coli str. 'clone D i2']
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T27526 NC_017651:4160050-4160475 [Escherichia coli str. 'clone D i2']
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCTCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT27526 WP_000939437.1 NC_017651:4159684-4160019 [Escherichia coli str. 'clone D i2']
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT27526 NC_017651:4159684-4160019 [Escherichia coli str. 'clone D i2']
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6