Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 505186..505822 Replicon chromosome
Accession NZ_CP120600
Organism Bacillus subtilis strain PRO112

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P5661_RS02585 Protein ID WP_003156187.1
Coordinates 505472..505822 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag P5661_RS02580 Protein ID WP_003225183.1
Coordinates 505186..505467 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5661_RS02560 (P5661_02560) 501546..502145 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
P5661_RS02565 (P5661_02565) 502240..502605 + 366 WP_003234281.1 holo-ACP synthase -
P5661_RS02570 (P5661_02570) 502771..503787 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
P5661_RS02575 (P5661_02575) 503901..505070 + 1170 WP_015252766.1 alanine racemase -
P5661_RS02580 (P5661_02580) 505186..505467 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P5661_RS02585 (P5661_02585) 505472..505822 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P5661_RS02590 (P5661_02590) 505938..506762 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
P5661_RS02595 (P5661_02595) 506767..507132 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
P5661_RS02600 (P5661_02600) 507136..507537 + 402 WP_003225192.1 serine/threonine-protein kinase RsbT -
P5661_RS02605 (P5661_02605) 507549..508556 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
P5661_RS02610 (P5661_02610) 508625..508954 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
P5661_RS02615 (P5661_02615) 508951..509433 + 483 WP_021481702.1 anti-sigma B factor RsbW -
P5661_RS02620 (P5661_02620) 509399..510187 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
P5661_RS02625 (P5661_02625) 510187..510786 + 600 WP_021481701.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T275243 WP_003156187.1 NZ_CP120600:505472-505822 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT275243 WP_003225183.1 NZ_CP120600:505186-505467 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References