Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 100424..101060 Replicon chromosome
Accession NZ_CP120598
Organism Bacillus subtilis strain PRO115

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag P5622_RS00605 Protein ID WP_003156187.1
Coordinates 100424..100774 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag P5622_RS00610 Protein ID WP_003225183.1
Coordinates 100779..101060 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5622_RS00565 (95468) 95468..96067 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
P5622_RS00570 (96067) 96067..96855 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
P5622_RS00575 (96821) 96821..97303 - 483 WP_003234299.1 anti-sigma B factor RsbW -
P5622_RS00580 (97300) 97300..97629 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
P5622_RS00585 (97691) 97691..98698 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
P5622_RS00590 (98710) 98710..99111 - 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
P5622_RS00595 (99115) 99115..99480 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
P5622_RS00600 (99485) 99485..100309 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
P5622_RS00605 (100424) 100424..100774 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
P5622_RS00610 (100779) 100779..101060 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
P5622_RS00615 (101176) 101176..102345 - 1170 WP_003234284.1 alanine racemase -
P5622_RS00620 (102460) 102460..103476 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
P5622_RS00625 (103642) 103642..104007 - 366 WP_003234281.1 holo-ACP synthase -
P5622_RS00630 (104102) 104102..104701 + 600 WP_003246687.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T275234 WP_003156187.1 NZ_CP120598:c100774-100424 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT275234 WP_003225183.1 NZ_CP120598:c101060-100779 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References