275231

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1346971..1347887 Replicon chromosome
Accession NZ_CP120577
Organism Bacillus subtilis strain PRO53

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag P5628_RS07155 Protein ID WP_003244695.1
Coordinates 1347141..1347887 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag P5628_RS07150 Protein ID WP_003232646.1
Coordinates 1346971..1347141 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5628_RS07115 (P5628_07115) 1343833..1344159 + 327 WP_277709537.1 XkdW family protein -
P5628_RS07120 (P5628_07120) 1344156..1344320 + 165 WP_014479563.1 XkdX family protein -
P5628_RS07125 (P5628_07125) 1344367..1345206 + 840 WP_124073160.1 phage-like element PBSX protein XepA -
P5628_RS07130 (P5628_07130) 1345259..1345528 + 270 WP_014479565.1 hemolysin XhlA family protein -
P5628_RS07135 (P5628_07135) 1345541..1345804 + 264 WP_014479566.1 phage holin -
P5628_RS07140 (P5628_07140) 1345817..1346710 + 894 WP_021479459.1 N-acetylmuramoyl-L-alanine amidase -
P5628_RS07145 (P5628_07145) 1346748..1346885 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
P5628_RS07150 (P5628_07150) 1346971..1347141 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
P5628_RS07155 (P5628_07155) 1347141..1347887 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
P5628_RS07160 (P5628_07160) 1347997..1348998 - 1002 WP_003232642.1 inorganic phosphate transporter -
P5628_RS07165 (P5628_07165) 1349011..1349628 - 618 WP_003218470.1 DUF47 domain-containing protein -
P5628_RS07170 (P5628_07170) 1349904..1351220 - 1317 WP_042974295.1 serine/threonine exchanger -
P5628_RS07175 (P5628_07175) 1351609..1352559 + 951 WP_015252261.1 ring-cleaving dioxygenase -
P5628_RS07180 (P5628_07180) 1352668..1352769 + 102 Protein_1357 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1311504..1356288 44784


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T275231 WP_003244695.1 NZ_CP120577:c1347887-1347141 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT275231 WP_003232646.1 NZ_CP120577:c1347141-1346971 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References